Anti-Inflammatory and Anti-Angiogenic Properties of Resveratrol in Ocular Diseases

Trans-resveratrol (Resv) was acquired from Sigma-Aldrich (St. Louis, MO, USA). It was tested using the COX fluorescent inhibitor screening assay kit (Cayman, Ann Arbor, MI, USA) according to the authors of Reference [1]. Scalar concentrations (specified in the graphs) of Resv were tested on purified, ovine COX-1 and human recombinant COX-2. The commercial inhibitors SC-560 and DuP-697 active on COX-1 and COX-2 isozymes, respectively, were used as controls. The fluorescent product of the reaction (Resorufin; excitation wavelength 530–540 nm; emission wavelength 585–595 nm) was quantified using a fluorimeter (Fluostar Omega, BMG labtech, Ortemberg, Germany).